Rapid intraoperative method for the identification of metastatic lymph
nodes from thyroid carcinoma
In this study, we established a rapid method for intraoperative
identification of thyroid carcinoma metastatic lymph nodes. We developed
a rapid method of thyroid globulin (Tg) detection called Tg-POCT based
on time-resolved fluorescence immunoassay and validated reagent
performance. Thyroid and lymph node tissues with metastatic thyroid
cancer were considered as the positive group, and muscle, fiber, fat,
and other tissues were considered as the negative control group to
verify the above method combined with fine needle puncture. CV of the
same batch number was 8.38% and that of different batch numbers was
11.24%. The minimum detection limit was 0.02 ng/mL. The test conformity
of tissue samples was 100% by using the self-designed reference value.
Thus, Tg-POCT is feasible as a rapid intraoperative method to identify
thyroid cancer metastatic lymph nodes.