Microwave assisted hydrodistillation (MAHD) and conventional hydrodistillation techniques were compared in the extraction of essential oils from Amomum subulatum seeds. The time required for MAHD method (70 min) is lesser than that for HD method (4 hr). There is a slight increase in the yield of extracted oil in MAHD method (3.35%) compared to HD (3%). GC-MS results show that MAHD extracted essential oil was wealthier in oxygenated compounds. 1, 8-Cineole was found to be a major compound in case of both the essential oil, followed by α-pinene. In MAHD the percentage of the major oxygenated monoterpene (1, 8- cineol) slightly increases from 88% to 89% as compared to hydrodistillation. Contrarily to this, the percentage of monoterpene hydrocarbon were decreased in MAHD than HD extracted oil. MAHD and HD extracted oils show good antibacterial activities against gram-negative and gram-positive bacteria. MAHD extracted oil shows better antibacterial activity than HD extracted against both gram positive and gram-negative bacteria. 2, 2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging antioxidant activities show that MAHD extract has better inhibition percentage than HD extract, and the IC50 value of MAHD was less than HD extracted oil.