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Nonionic detergent micelle aggregates: an economical alternative to Protein A chromatography
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  • Guy Patchornik,
  • Gunasekaran Dhandapani,
  • Ellen Wachtel,
  • Mordechai Sheves
Guy Patchornik
Ariel University Faculty of Natural Sciences

Corresponding Author:[email protected]

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Gunasekaran Dhandapani
Ariel University Faculty of Natural Sciences
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Ellen Wachtel
Weizmann Institute of Science Department of Biological Chemistry
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Mordechai Sheves
Weizmann Institute of Science Faculty of Chemistry
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We have recently described a non-chromatographic, ligand-free approach for antibody (Ab) purification based on specially designed: [Tween-20:bathophenanthroline:Fe2+] aggregates. To assess the potential generality of this approach, a variety of detergents belonging to four nonionic detergent families (Tween, Brij, Triton and Pluronic) have now been studied. All surfactant aggregates lead to high purity of the recovered Abs (>95%, by gel densitometry). Good overall Ab recovery yields were observed with: Tween-20 (80-83%), Brij-O20 (85-87%) and Triton X-100 (87-90%), while Pluronic F-127 was less efficient (42-53%). Of additional importance is the finding that the process can depend on filtration (rather than centrifugation), thereby allowing a continuous purification mode that leads to the recovery of monomeric IgG’s (by DLS) and preservation of Ab specificity (by ELISA). The amphiphilic chelator, bathophenanthroline (batho) is recycled almost quantitatively (95%) by crystallization. Good IgG recovery yields (~80%) are also observed when Ab concentrations are increased from 1 mg/ml to 3-5 mg/mL. Potential advantages of the purification platform for industrial downstream processing of therapeutic monoclonal antibodies (mAbs), are discussed.
18 Feb 2020Submitted to Biotechnology and Bioengineering
18 Feb 2020Submission Checks Completed
18 Feb 2020Assigned to Editor
21 Apr 2020Reviewer(s) Assigned
26 May 2020Review(s) Completed, Editorial Evaluation Pending