Nutrient bioavailability and uptake by a cyanobacteria consortium
cultivated at high pH and alkalinity
Abstract
Alkaliphilic microalgae and cyanobacteria have gained significant
importance due to their robustness, high biomass productivity, and
ability to efficiently capture carbon dioxide directly from the
atmosphere. To grow these alkaliphiles under high pH (pH
>10.4) and high alkalinity conditions (0.1 - 0.5 M),
substantial amounts of nutrients are required, which could potentially
increase the operating costs of cultivation and adversely affect its
environmental footprint. One conceivable way of tackling this issue is
by re-using the spent medium and supplementing only the depleted
nutrients. To effectively re-use the spent medium, first it is important
to understand the nutrient bioavailability and uptake by alkaliphiles.
In this study, we have determined the bioavailability of nutrients (e.g.
C, N, P, S, Mg, S, Ca, Fe, etc.) in a high pH (> 10.4) and
alkalinity (0.5 M) medium. Our results show that –with the exception of
Mg, Ca, and Fe– all the nutrients are in bioavailable form for
microbial growth. The availability of Mg, Ca, and Fe is limited because
of precipitate formation with carbonates and hydroxides. Additionally,
we have also carried out cultivation experiments to determine biomass
productivity, elemental composition, and stoichiometric formula based on
nutrient uptake. The cyanobacterial cultures grew well without any
inhibition and a maximum productivity of 153 mg-AFDW
L-1 d-1 was achieved. The elemental
composition of biomass suggested that Mg and Ca content in biomass is
low, consistent with the limited availability of these elements during
the growth. Finally, the derived stoichiometric equation resulted in the
following chemical formula
CH1.75N0.17O0.41P0.003.