Development of an in vitro Callus Induction Protocol and Shoot
Proliferation for Selected Jatropha (Jatropha curcas) Accessions
Abstract
Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical
and subtropical parts of the world. The present study was undertaken to
establish a protocol for in vitro callusing of three Jatropha accessions
namely; Metema, Adami Tulu and Shewa Robit accession from leaf explants.
The experiment was laid out in CRD with five replications in factorial
arrangement. The medium supplemented with combination of 1.0 mg/L BAP
and 1.0 mg/L 2,4-D resulted in maximum percentage of callus (100%)
formed for all accessions. The maximum shoot regeneration (66.67%) from
callus with 10.13 number of shoot was obtained from Shewa Robit
accession in MS medium fortified with TDZ (0.5 mg/L) and IBA (0.1 mg/L).
The presence of TDZ in the shoot regeneration medium has greater
influence on the induction of adventitious shoot buds, whereas MS
supplemented with BAP alone and combination with IBA did not induce
shoot regeneration from callus culture. The results obtained in the
present study would facilitate the high callus induction and
regeneration responses in Jatropha for its improvement using
biotechnological tools.