A Platform to Quantify Phenotypic Responses to Root-Root Interactions
Among Kin and Non-kin Common Beans
Abstract
Quantifying phenotypes of root-root interactions would allow a greater
understanding of how plants react to belowground competition through
plasticity of architectural traits. Past research has shown that plants
will over proliferate roots in the presence of competition, leaving less
resources to allocate above ground, negatively impacting shoot growth
and yields [1]. Further evidence highlights plants may recognize kin
and non-kin relationships, responding by avoiding competing with close
relatives and instead allocating root mass closer to non-kin neighbors
[2]. In an attempt to visualize and quantify root architecture
plasticity involved in these root-root interactions, we developed a
modified mesocosm system. Within the mesocosm box common bean seeds were
germinated 10 inches apart from each other. Mesh screens were placed on
either side of each bean, in order to capture root growth towards each
other and/or away from each other. Two treatments were involved, with
mesocosms grown with either kin or non-kin plants. Plants will be
harvested at the 6-week mark, when the root archetype will be developed
and prominent. During harvesting one side of the mesocosm box will be
removed and the soil will be washed away. We will quantify the degree of
root competition by counting the number of roots passing the mesh
screen. All general 2D root traits will be measured in DIRT2D. This
experiment will shine light on an understudied section of crop science
and will allow farmers and researchers a better understanding of an
otherwise unseen phenomena.