Constraining the subsurface thermal regime is of importance not just to the offshore hydrocarbon exploration industry but also for understanding the geothermal regime in context of microbiological activity. This regime is tested through the use of seismic data to noninvasively estimate subsurface temperatures through the identification of bottom simulating reflectors (BSRs) at the base gas hydrate stability zone (GHSZ) to mark out an isotherm. This reflection seismic thermometry methodology is applied to the Blake Ridge, offshore east coast USA, where ODP Leg 164 boreholes provide temperature data required for thermal model constraints. 3D thermal modelling using reflection seismic data is used to examine the lateral variability of the shallow thermal regime while 1D thermal modelling is applied to ODP Sites 994, 995 and 997. The resulting estimated subsurface temperature profiles had a margin of error of 13.9% compared to in situ temperature measurements recorded from the boreholes. This method of thermal modelling could significantly expand the application of thermal data from ODP sites to areas covered by seismic data, which would considerably benefit researchers worldwide in industry and academia.