Background and purpose: Studies have shown that some cytokines of COVID-19 were elevated. This study aims to assess whether IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 serve as diagnostic biomarkers of COVID-19 and offer prognostic insight upon initial presentation to help guide treatment,In addition, the relationships between them and gender, age, antibody concentration and course of disease were also discussed. Methods: The serum levels of cytokines above in experience group (COVID-19 patients) and control group (other diseases patients and healthy people) were detected by ELISA. Results: Most of the serum level of cytokines above in experience group were significantly higher than those in control group, and AUCs of COVID-19 diagnosed by they were 0.735, 0.775, 0.595, 0.821, 0.848, 0.387 and 0.987. The serum levels of some cytokines in male patients had noticeably higher than those in female patients, while the serum levels of almost all cytokines of the elderly were higher than that of the youth and middle-aged patients. The serum levels of IP-10 in patients were positively correlated with IgM, while TNF-α were negatively correlated with IgG. The levels of IL-1 β and IL-6 increased sharply in the early stage of COVID-19, then decreased gradually; the levels of IL-10, MCP-1, TNF-α and IL-4 increased sharply in the middle stage, while the levels of IP-10 increased sharply in the late stage. Conclusion: The cytokines above can prove to be great significance for clinical diagnostics of COVID-19, and the levels of cytokines in patients have some relationships with gender, age and course of disease.