Cytology
Three hundred microliters of fluid from TW and BALF (left, right and pooled) were cytocentrifuged (80g , 10 min) and stained with May-Grünwald-Giemsa (MGG). A differential cell count was performed on 400 cells and the number of each cell type was recorded as a percentage of total nucleated cells, excluding epithelial cells. The presence of fungal particles (spores and hyphae, extracellular or phagocytosed) and bacteria was documented.