Cytology
Three hundred microliters of fluid from TW and BALF (left, right and
pooled) were cytocentrifuged (80g , 10 min) and stained with
May-Grünwald-Giemsa (MGG). A differential cell count was performed on
400 cells and the number of each cell type was recorded as a percentage
of total nucleated cells, excluding epithelial cells. The presence of
fungal particles (spores and hyphae, extracellular or phagocytosed) and
bacteria was documented.