3.3 GR mediates the downregulation of DOT1L and its
target gene by Dex in B lymphoma cells
Dex suppressed the viability of Raji cells with apparent
concentration-dependent effect (Figure 3A). However, Dex did not inhibit
the growth of THP-1 cells even at a high concentration (8 μM). Moreover,
Dex dramatically reduced the mRNA level of DOT1L and its target
gene MEIS1 in Raji cells (Figure 3B). In addition, Dex remarkably
downregulated the level of H3K79 in Dex-sensitive Raji cells but not in
the Dex-insensitive THP-1 cells (Figure ). Pretreatment with GR
antagonist RU486 significantly mitigated the Dex-induced suppression of
cell growth, the mRNA levels of DOT1L , and MEIS1 , and
protein levels of H3K79 in Raji cells (Figure 3D-F). Together, Dex could
downregulate DOT1L and its target gene in a GR-dependent manner
for B lymphoma cells.