3. Results
3.1
DOT1L is highly-expressed in B lymphoma cells
To investigate the expression difference of DOT1L between normal tissues
and hematological malignancies, the TCGA database was used. As
demonstrated in Figure 1A, DOT1L expression was higher in hematopoietic
and lymphoid tumors than normal tissues. Moreover, the basal expression
of H3K79 (the downstream effector of DOT1L) was examined in several B
lymphoma cells. The
MLL-rearranged
cell line MV4-11 was known to express a high level of DOT1L/H3K79 and
used as a positive control [18]. As illustrated in Figure 1B, H3K79
was relatively highly expressed in three of the four B lymphoma cells
(Burkitt’s lymphoma
cells
Raji, Namalwa, Daudi, and mantle cell lymphoma cell Jeko-1), but not the
acute
monocytic leukemia cell (THP-1). Taken together, the above results
indicated that DOT1L might contribute to the initiation and development
of B lymphoma.