Figure 4. Dex decreases mRNA level ofDOT1L
through weakening its mRNA stability. (A) Outline of the DOT1Lpromoter region (-2800bp to +200 bp) containing the putative GR binding
sites. The -1800 bp to +200 bp region was fused with pGL3-Basic vector
to obtain
pGL3-DOT1L.
(B) Cells were transfected with pGL3-DOT1L or pGL3-Basic (control
vector) in the presence of DMSO, then treated with Dex or RU486, and the
Dual-Luciferas Reporter System was used to detect the relative
luciferase activity. (C) Raji cells were incubated with Act D in the
presence or absence of Dex for the indicated times, and the RT-qPCR was
utilized to detect the mRNA level of DOT1L. (D) The quantitative
representation of the results of (C).