The Hoxb8 MAT performs similar as the BAT in clinical
utility assessment.
Further, we directly compared the diagnostic performance of the Hoxb8
MAT to the BAT, SPT and sIgE measurement all using peanut extract as an
allergen source. While a mixed effect model integrating 10 and 100 ng/mL
of peanut allergen stimulus has previously been reported to be most
accurate for the BAT (AUROC 0.975) , we here used a logistic regression
model taking into consideration all peanut allergen concentration
(0.01-1000 ng/mL) as variables for the Hoxb8 MAT as well as for the BAT.
In this analysis the Hoxb8 MAT and the BAT outperformed SPT and sIgE
measurements (Fig. 5 and Table S2 ). Both cellular
assays (i.e. Hoxb8 MAT and BAT) were highly accurate with similar
diagnostic performance (AUROC 0.95 and 0.96, respectively).