RNA extraction and RT-qPCR Analysis:
Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to assess the expression of the ARG1 gene and NOS1 gene in serum using specific primers. The extraction of total RNA was carried out using the Steady Pure Universal RNA Extraction Kit obtained from Accurate Biotechnology, Hunan Co., Ltd. Reverse transcription was performed using the Evo M-MLVRT Kitt kit from the same manufacturer. Subsequently, qRT-PCR was conducted on the CFX96 Real-Time System by Bio-Rad, utilizing the SYBR Green Supermix provided by Accurate Biotechnology, Hunan Co., Ltd. The 2-ΔΔCt method was employed to calculate the relative gene expression levels, with the housekeeping gene GAPDH serving as the internal control. The designed primers are listed in Table S1.